Spectroscopic method for degradation study of Glibenclamide was described. Force degradation study is a process in which natural degradation rate of pharmaceutical product is increased by application of an additional stress. Glibenclamide is second generation sulphonyl urea derivative and used as Antidiabetic agent. To develop and validate a simple and precise stability indicating UV spectroscopic method for the estimation of Glibenclamide in bulk and tablet dosage form. Force degradation study of Glibenclamide was done by High performance liquid chromatography method but not still done by UV Spectroscopy. Separation of Glibenclamide from its degradation product were achieved by UV spectroscopic method using ethanol. Glibenclamide was subjected to stress condition such as hydrolysis (acid and base), oxidation, photolysis. The maximum absorbance was found to be at 230.6 nm and found to be linear over the range 5-25 ug/ml with good correlation coefficient ( r2) 0.9980. The limit of detection and quantification were 1.1041 and 3.3460 ug/ml respectively. Major degradation was observed in acid, alkaline and oxidative conditions. Glibenclamide was quietly stable under thermal condition. Thus the proposed method was found to be economical, selective and sensitive for desirable range.
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